Gene Editing & Detection Analysis
Service Overview
Provides comprehensive gene detection and analysis services through advanced molecular biology techniques, offering precise gene-level data support for efficacy evaluation, target validation, and biomarker discovery. The center possesses high-throughput, high-sensitivity, and high-accuracy detection capabilities to meet gene analysis needs from basic research to preclinical development stages. Our center is equipped with a professional technical team and a sound quality management system to ensure the accuracy and reliability of test results. We provide clients with high-quality gene detection and analysis services, supporting the precision and personalization of drug development.
Service Overview
Provides comprehensive gene detection and analysis services through advanced molecular biology techniques, offering precise gene-level data support for efficacy evaluation, target validation, and biomarker discovery.
The center possesses high-throughput, high-sensitivity, and high-accuracy detection capabilities to meet gene analysis needs from basic research to preclinical development stages.
Our center is equipped with a professional technical team and a sound quality management system to ensure the accuracy and reliability of test results. We provide clients with high-quality gene detection and analysis services, supporting the precision and personalization of drug development.
Service Content
1.Gene Editing & Cell Model Services
Gene Editing Services: CRISPR/Cas9-mediated gene knockout, knock-in, point mutation, humanization.
Primary Cell Gene Editing: Genetic manipulation in more clinically relevant primary cell models.
2.Gene-Level Efficacy Evaluation
Gene Expression Analysis.
Gene Polymorphism & Metabolism Assessment.
Epigenetic Analysis.
Gene Function & Safety Assessment.
3.Gene Function & Safety Assessment
Target Function Verification.
Genetic Toxicity Testing.
Biodistribution of Nucleic Acid Therapeutics.
Off-target Effect Detection.
Replication-Competent Virus Testing.
Technical Means
(1) Detection Technology Platforms
1.Real-time Quantitative PCR Systems: Utilizing TaqMan probe and SYBR Green methods for high-sensitivity gene expression quantification.
2.High-throughput Sequencing Platforms: Illumina NovaSeq, etc., supporting whole-genome sequencing, exome sequencing, transcriptome sequencing, etc.
3.Digital PCR Systems: Providing absolute quantitative analysis, detecting low-abundance mutations and copy number variations.
4.Gene Chip Platforms: For large-scale gene expression profiling and SNP genotyping analysis.
(2) Analytical Technologies
5.Multiplex PCR Technology: Simultaneous detection of multiple gene loci.
6.Pyrosequencing Technology: For precise quantification of DNA methylation levels.
7.Chromatin Immunoprecipitation (ChIP) Technology: Studying protein-DNA interactions.
8.Single-cell Sequencing Technology: Resolving gene expression heterogeneity at the single-cell level.
(3) Bioinformatics Analysis Pipeline
9.Raw Data Quality Control: Data cleaning using tools like FastQC, Trimmomatic.
10.Sequence Alignment: Sequence alignment using software like STAR, HISAT2.
11.Differential Analysis: Statistical analysis using R packages like DESeq2, edgeR.
12.Functional Annotation: Functional enrichment analysis using databases like DAVID, Metascape.
13.Network Analysis: Molecular network construction using tools like Cytoscape.
